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phospho synapsin1 ser603  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phospho synapsin1 ser603
    FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) <t>pSynapsinSer603/Synapsin1,</t> (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.
    Phospho Synapsin1 Ser603, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 16 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phospho+synapsin1+ser603/Phospho-Synapsin-1+(Ser605)+Rabbit+mAb/pm39653030-70-41-44
    Average 93 stars, based on 16 article reviews
    phospho synapsin1 ser603 - by Bioz Stars, 2026-10
    93/100 stars

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    1) Product Images from "Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats."

    Article Title: Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats.

    Journal: British journal of pharmacology

    doi: 10.1111/bph.17403

    FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) pSynapsinSer603/Synapsin1, (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.
    Figure Legend Snippet: FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) pSynapsinSer603/Synapsin1, (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.

    Techniques Used: Western Blot

    Related Articles

    Western Blot:

    Article Title: Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats.
    Article Snippet: Blots were blocked for 1 h at room temperature with I-Block solution (Life Technologies Italia, Italy) in TBS 0.1% Tween-20 buffer and incubated with antibodies against the proteins of interest.. The conditions of the primary antibodies were the following:- anti-GLT-1 (1:2000, Abcam, Cat# Ab41621, RRID:AB_ 941782), anti-vGluT1 (1:1000, Cell Signalling Technology, Cat# 12331, RRID:AB_2797887), anti vGluT2 (1:1000, Cell Signalling Technology, Cat# 71555, RRID:AB_2799805), anti xCT [SLC7A11] (1:1000, Abcam, Cat# ab216876), anti phospho-Synapsin1 Ser603 (1:1000, Cell Signalling Technology, Cat# 88246, RRID:AB_2800119), anti Synapsin1 (1:2000, Santa Cruz Biotechnology, Cat# sc-8295, RRID:AB_677472), anti phospho-αCaMKII Thr286 (1:1000, Thermo Fisher Scientific, Cat# MA1047, RRID:AB_325402), anti αCaMKII (1:2000, Millipore, Cat# 05-532, RRID:AB_309787), anti GluN1 (1:1000, Cell Signalling Technology, Cat# 5704, RRID:AB_1904067), anti GluN2A (1:1000, Cell Signalling Technology, Cat# 4205, RRID: nloaded from https://bpspubs.onlinelibrary.w iley.com /doi/10.1111/bph.17403 by IN A SP - N E PA L , W iley O nline L ibrary on [13/12/2024].. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense AB_2112295), anti GluN2B (1:1000, Cell Signalling Technology, Cat# 14544, RRID:AB_2798506), anti SAP102 (1:1000, Cell Signalling Technology, Cat# 47421, RRID:AB_2799325), anti Rabphilin (1:2000, Proteintech, Cat# 11396-1-ap, RRID:AB_2181145), anti-PSD95 (1:2000, Cell Signalling Technology Cat# 3450, RRID:AB_2292883), anti phospho-CREB Ser133 (1:1000, Cell Signalling Technologies, Cat# 9198, RRID:AB_2561044), anti CREB (1:1000, Cell Signalling Technologies, Cat# 9197, RRID:AB_331277), anti phospho-NL-1 Thr739 (1:1000, Sigma-Aldrich, Cat# ABN1707), anti-NL-1 (1:1000, Synaptic System, Cat# 129003, RRID:AB_887746), anti-Arc/Arg3.1 (1:500, BD Biosciences, Cat# 612603, RRID:AB_399886) and anti β-actin (1:5000, Sigma-Aldrich, Cat# A5441, RRID:AB_476744).



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    FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) <t>pSynapsinSer603/Synapsin1,</t> (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.
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    FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) <t>pSynapsinSer603/Synapsin1,</t> (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.
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    FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) <t>pSynapsinSer603/Synapsin1,</t> (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.
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    FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) <t>pSynapsinSer603/Synapsin1,</t> (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.
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    Image Search Results


    FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) pSynapsinSer603/Synapsin1, (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.

    Journal: British journal of pharmacology

    Article Title: Memantine-induced functional rewiring of the glutamate synapse in the striatum of dopamine transporter knockout rats.

    doi: 10.1111/bph.17403

    Figure Lengend Snippet: FIGURE 1 Presynaptic changes in the striatum of DAT+/+ and DAT/ rats. Protein levels of (a) VGLUT1, (b) VGLUT2, the ratio of (d) pSynapsinSer603/Synapsin1, (e) GLT-1 and (f) xCT were measured in the whole homogenate of the striatum of DAT+/+ and DAT/ rats. The ratio (c) pαCaMKIIThr286/αCaMKII was investigated in the postsynaptic density. (g) Representative immunoblots are shown for VGLUT1, VGLUT2, pαCaMKIIThr286, αCaMKII, pSynapsinSer603, Synapsin1, GLT-1, xCT and β-actin. Results are expressed as mean percentage ± mean standard error from five independent determinations for each experimental group. Unpaired Student's t test *P < 0.05 versus DAT+/+ rats.

    Article Snippet: The conditions of the primary antibodies were the following:- anti-GLT-1 (1:2000, Abcam, Cat# Ab41621, RRID:AB_ 941782), anti-vGluT1 (1:1000, Cell Signalling Technology, Cat# 12331, RRID:AB_2797887), anti vGluT2 (1:1000, Cell Signalling Technology, Cat# 71555, RRID:AB_2799805), anti xCT [SLC7A11] (1:1000, Abcam, Cat# ab216876), anti phospho-Synapsin1 Ser603 (1:1000, Cell Signalling Technology, Cat# 88246, RRID:AB_2800119), anti Synapsin1 (1:2000, Santa Cruz Biotechnology, Cat# sc-8295, RRID:AB_677472), anti phospho-αCaMKII Thr286 (1:1000, Thermo Fisher Scientific, Cat# MA1047, RRID:AB_325402), anti αCaMKII (1:2000, Millipore, Cat# 05-532, RRID:AB_309787), anti GluN1 (1:1000, Cell Signalling Technology, Cat# 5704, RRID:AB_1904067), anti GluN2A (1:1000, Cell Signalling Technology, Cat# 4205, RRID: nloaded from https://bpspubs.onlinelibrary.w iley.com /doi/10.1111/bph.17403 by IN A SP - N E PA L , W iley O nline L ibrary on [13/12/2024].

    Techniques: Western Blot